| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138794 |
| Chromosome: | chromosome 3 |
| Location: | 8683601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203793 | GT90-35,GT90F35 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 35 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTGCATGCGACGGCAACCCCAAGGTCA |
| Internal bar code: | TATGAGGTTAGGAGCCCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 97.7728 |
| LEAP-Seq n confirming: | 878 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCATTTGAGTGGACTT |
| Suggested primer 2: | CTGCTGGTCAGATGGTCAGA |