Insertion junction: LMJ.RY0402.138801_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.138801
Chromosome:chromosome 2
Location:2188506
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g089608 (1 of 1) K11136 - regulator of telomere elongation helicase 1 [EC:3.6.4.12] (RTEL1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCCTCCCCCCGCGCACCCACCAGCTG
Internal bar code:CCGGTGTCGAGTTGTTCTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:348
LEAP-Seq percent confirming:99.596
LEAP-Seq n confirming:493
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGGTGTGTGCCTATGTG
Suggested primer 2:TTTGGTTTAACTTGGGCTGG