| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138815 |
| Chromosome: | chromosome 16 |
| Location: | 3575123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681657 | (1 of 1) PF01344//PF13415//PF13418 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGACACCGCCTCGTACACATGGACCGT |
| Internal bar code: | GCGCTGCAGCAGTAGTGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.8139 |
| LEAP-Seq n confirming: | 5364 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTGAAGAAACAGAACC |
| Suggested primer 2: | TCATGATCAGGGAGGTAGGC |