| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138819 |
| Chromosome: | chromosome 6 |
| Location: | 7377326 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTAGGGCCTGGCCCGCAGCACGCAGGC |
| Internal bar code: | CCTGGAGTACTCCGCCTTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1291 |
| LEAP-Seq percent confirming: | 99.8894 |
| LEAP-Seq n confirming: | 903 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTCTGGAGTGTTTTGA |
| Suggested primer 2: | CACAAGAGGAGGAGGACGAG |