| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138840 |
| Chromosome: | chromosome_9 |
| Location: | 3671336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390615 | FAP12 | Triacylglycerol lipase and Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTAAAATGGTTACAATTCCCTAGCGTAGC |
| Internal bar code: | GACGAAAAGGTTTAAGGAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.6505 |
| LEAP-Seq n confirming: | 8268 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCGTACAAAGGGTCAC |
| Suggested primer 2: | CACACCTACCAGCTCCCACT |