| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138858 |
| Chromosome: | chromosome 6 |
| Location: | 860261 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254800 | SPL15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATGGCTGATGCTGCTGTACCCTGGCAT |
| Internal bar code: | ACGCAGGGGCGGTTTCTCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1196 |
| LEAP-Seq percent confirming: | 96.6049 |
| LEAP-Seq n confirming: | 11154 |
| LEAP-Seq n nonconfirming: | 392 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTAGCTGCATTCCCGAG |
| Suggested primer 2: | CCGGTAGGTACTGGTAGGCA |