| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138878 |
| Chromosome: | chromosome 9 |
| Location: | 157651 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386735 | DLA1 | Dihydrolipoamide acetyltransferase; (1 of 1) PTHR23151//PTHR23151:SF9 - DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED // DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCGCGAATGCCCGTACAGCACTTACCA |
| Internal bar code: | GCAGAAACATCCGTTTAAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 99.6501 |
| LEAP-Seq n confirming: | 18225 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGTCGGTGAACGACTTT |
| Suggested primer 2: | GTTACAAAATGGCTGCGGTT |