| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138880 |
| Chromosome: | chromosome 4 |
| Location: | 617470 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217943 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTATCAACCCCGCCCCCCTCGCCCGCTC |
| Internal bar code: | GCAACCATGGGGATAATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 87.3508 |
| LEAP-Seq n confirming: | 366 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCTCAAGCAAGTAAG |
| Suggested primer 2: | GCGGAGTGTGTGTACGCTAA |