| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138881 |
| Chromosome: | chromosome 3 |
| Location: | 1265933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150101 | NRT2D | Nitrate/nitrite transporter; (1 of 5) PTHR23515//PTHR23515:SF2 - FAMILY NOT NAMED // HIGH AFFINITY NITRATE TRANSPORTER 2.3-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGTGGCTGATGTGGCTGCTGCAGGGGG |
| Internal bar code: | GTGCGGCGGGTGTCTGCCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1125 |
| LEAP-Seq percent confirming: | 98.3599 |
| LEAP-Seq n confirming: | 76405 |
| LEAP-Seq n nonconfirming: | 1274 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACGGTGTGATGGTGTAG |
| Suggested primer 2: | TCCTGCTATTTGCGACACAG |