| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138883 |
| Chromosome: | chromosome 10 |
| Location: | 2512753 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436400 | (1 of 2) PTHR13271//PTHR13271:SF11 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGTGAGGGCTTCAACTGTAGATGTAT |
| Internal bar code: | CTGGACCCTAACAAACGGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 98.4648 |
| LEAP-Seq n confirming: | 13918 |
| LEAP-Seq n nonconfirming: | 217 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGACTGTGTGGGAGG |
| Suggested primer 2: | GACTGCGTCATACGCGACTA |