| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138901 |
| Chromosome: | chromosome 8 |
| Location: | 379629 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358573 | MLO3 | transient receptor potential ion channel protein; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAGTAAGGCGGCAGGAGCCTATCTAGT |
| Internal bar code: | AGGAGCTTTTTCGCAGTTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1061 |
| LEAP-Seq percent confirming: | 99.5757 |
| LEAP-Seq n confirming: | 5398 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATGAAGGTCACTAACA |
| Suggested primer 2: | GCACATATGCCTTGGGAACT |