| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138908 |
| Chromosome: | chromosome_6 |
| Location: | 1198474 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257600 | FA1 | Flagellar Autonomy Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCCCACTCACATTGCCGCCGCAGTTGGAA |
| Internal bar code: | ATACTGTCCCAGACGTAAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 98.7234 |
| LEAP-Seq n confirming: | 696 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTGGCTGCTGTGTTTGA |
| Suggested primer 2: | CTGCCATATGACACTGGTGC |