| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138942 |
| Chromosome: | chromosome 3 |
| Location: | 619329 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145707 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTGAGCGTGGGGGCTGAGCGGTGTGT |
| Internal bar code: | GCGGAGAGCGGATGGATGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 78.1827 |
| LEAP-Seq n confirming: | 3218 |
| LEAP-Seq n nonconfirming: | 898 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACGGAATGCATCATCAG |
| Suggested primer 2: | TGGTCAGATGGAACGTGGTA |