| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138942 |
| Chromosome: | chromosome 4 |
| Location: | 3843318 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229948 | (1 of 2) 1.3.1.89 - tRNA-dihydrouridine(47) synthase (NAD(P)(+)) / tRNA-dihydrouridine synthase 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAGGGGGTGTCACCGCCCCACACAGCCC |
| Internal bar code: | CCGAGACATACTCTGCTCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 181 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTACTGTCAGCTTCACG |
| Suggested primer 2: | GGACTGTGTATGCGGATGTG |