| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138955 |
| Chromosome: | scaffold_35 |
| Location: | 10444 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre35.g759247 | SRR5 | Scavenger receptor cysteine rich (SRCR) protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGAGCGAGCCGGGTGCGGGCGGCGCAGGT |
| Internal bar code: | GATCGCCTTAGGATGGTACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 951 |
| LEAP-Seq percent confirming: | 97.6979 |
| LEAP-Seq n confirming: | 8700 |
| LEAP-Seq n nonconfirming: | 205 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGATGAGGTGGATGAG |
| Suggested primer 2: | CTCGGTGCTGTTCCTAAAGC |