| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138959 |
| Chromosome: | chromosome 12 |
| Location: | 5457760 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530550 | KDG2 | (1 of 1) 2.7.1.91 - Sphinganine kinase / Dihydrosphingosine kinase; Diacylglycerol kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCACGTCGCAGAGTCGCGTTGCTCAA |
| Internal bar code: | AGAGGTTAGGAAGATGATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 99.6774 |
| LEAP-Seq n confirming: | 8033 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGTCATCACCTTGCCTC |
| Suggested primer 2: | AATTCCCACCCTCCTAATGG |