Insertion junction: LMJ.RY0402.138967_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.138967
Chromosome:scaffold 19
Location:201090
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre19.g751247 (1 of 1) K16742 - ADP-ribosylation factor-like protein 2-binding protein (ARL2BP, BART) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCTGCCTCATCCTCCTTTTAGCTTGGTT
Internal bar code:TGCCAAGGCGCATCATTGATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:218
LEAP-Seq percent confirming:97.8581
LEAP-Seq n confirming:731
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGACGAGGAGTTTGAGG
Suggested primer 2:ATGAACAGCTAGCCCCCTCT