| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138988 |
| Chromosome: | chromosome 5 |
| Location: | 3218784 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239900 | ARS15 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAATGTATGGGGCAGTCATCGCTGAG |
| Internal bar code: | CAGCTGTGATAGTTTCTGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGCATGTGTGTGTGTGA |
| Suggested primer 2: | ACGTCCTCCCATGCACTTAC |