| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138997 |
| Chromosome: | chromosome 8 |
| Location: | 73792 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358524 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAAAGTTGGGAGCTAATACGTTGGGCC |
| Internal bar code: | TGGATTCTGATCTAGGCCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 86.6492 |
| LEAP-Seq n confirming: | 331 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATTGAGGCCAACAACTC |
| Suggested primer 2: | GCTGCTGTTGGTCAGGTACA |