| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139026 |
| Chromosome: | chromosome 5 |
| Location: | 773096 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248400 | CAH4,mtCA1,MCA | Mitochondrial carbonic anhydrase, beta type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCTGCGGCCGTTCCCCCGCTTGCAGC |
| Internal bar code: | CCTCTTGAATCATGGGCTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 98.9074 |
| LEAP-Seq n confirming: | 1720 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGCCTCGCGAACTTAC |
| Suggested primer 2: | GAGAGAGGAGATGACGCCAG |