| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139033 |
| Chromosome: | chromosome 16 |
| Location: | 2892985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTCAAGTGCAGTGAGGCGACCCGGGC |
| Internal bar code: | CCGTAAGATTTCCTATTCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.6282 |
| LEAP-Seq n confirming: | 16615 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCGAATATCGAGAGCTG |
| Suggested primer 2: | ACGGTCCATGATATCCCAAA |