Insertion junction: LMJ.RY0402.139033_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139033
Chromosome:chromosome 17
Location:4351655
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g731250 uS15m,MRPS15 (1 of 1) K02956 - small subunit ribosomal protein S15 (RP-S15, MRPS15, rpsO); Mitochondrial ribosomal protein S15 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTCTCCATGGGATCAGCAATGTGGGC
Internal bar code:CCACAAACGCCGTGGAAAACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:772
LEAP-Seq percent confirming:98.8752
LEAP-Seq n confirming:5714
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCACACACACACTCGCAT
Suggested primer 2:TACGGGAGTACGGGAAGTTG