| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139039 |
| Chromosome: | chromosome 12 |
| Location: | 8494773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548000 | (1 of 1) K14550 - U3 small nucleolar RNA-associated protein 10 (UTP10, HEATR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGCAAAAACCCATGTCAAGCCCGTAC |
| Internal bar code: | CGAAGTTGTGAGTTGAGCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.796 |
| LEAP-Seq n confirming: | 5869 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCGTGAGCTTCATCGAC |
| Suggested primer 2: | CGGATGAGCTGTGTAGACGA |