| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139048 |
| Chromosome: | chromosome 1 |
| Location: | 120565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000900 | CPLD20 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGTCGGTGCTTGCCTTCATGCCGGGC |
| Internal bar code: | CTGACGACTGGGAAGAAGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 98.9754 |
| LEAP-Seq n confirming: | 2898 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTCTTGCGTGTCCCTAT |
| Suggested primer 2: | GCCCTTGACAAGATTGTGGT |