| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139052 |
| Chromosome: | chromosome 3 |
| Location: | 1385724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150800 | (1 of 45) IPR029052 - Metallo-dependent phosphatase-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACCATTATGCATGAACCTGCTCACCAC |
| Internal bar code: | GCTAAGCGCAGGTAGGTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 98.8711 |
| LEAP-Seq n confirming: | 2715 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCCATCCCTCACTCAAC |
| Suggested primer 2: | GAACCGCAAATGGATTCTGT |