| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139062 |
| Chromosome: | chromosome 6 |
| Location: | 7653572 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301550 | MFT18 | Major facilitator superfamily transporter; (1 of 3) PTHR23505//PTHR23505:SF21 - FAMILY NOT NAMED // SPHINGOLIPID TRANSPORTER SPINSTER HOMOLOG 1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGAGGGCGGGCGTCTCAGGCTCCTAG |
| Internal bar code: | GGTCTTTGTCGGCACGGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 99.695 |
| LEAP-Seq n confirming: | 11440 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTAGCTAACCTCCTGC |
| Suggested primer 2: | GTTCAGAGACTGAGGGGCTG |