| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139106 |
| Chromosome: | chromosome 10 |
| Location: | 154779 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418800 | DIV156 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAGGAGGAGGGGCATGACGTTACACTG |
| Internal bar code: | GGTACCAGTATATAAGATTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.505 |
| LEAP-Seq n confirming: | 2814 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATCCTCAGTCGCCTCAA |
| Suggested primer 2: | TCAACTACACGCTACAGCCG |