Insertion junction: LMJ.RY0402.139110_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.139110
Chromosome:chromosome 5
Location:883476
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g247500 (1 of 2) IPR001841//IPR013083//IPR018957 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, C3HC4 RING-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACTGATTCAGGCAATCATACTGTATGG
Internal bar code:CGAGAGAAGATGGGGTACGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:717
LEAP-Seq percent confirming:95.6171
LEAP-Seq n confirming:8530
LEAP-Seq n nonconfirming:391
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTGGCGGCGTAGAGATAC
Suggested primer 2:ATGAGTGTATTGCGTGCGAG