Insertion junction: LMJ.RY0402.139114_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139114
Chromosome:chromosome 1
Location:4430623
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g030100 PTK13 Protein tyrosine kinase; (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCACCCACGGCTAGCTGGACGCGTCAG
Internal bar code:GCCTTCCTGAAAGGATCGCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:186
LEAP-Seq percent confirming:1.19284
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1988
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTAACTGCGAGGCACATCAA
Suggested primer 2:CTGGAAATGGGTTGCCTTTA