Insertion junction: LMJ.RY0402.139124_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.139124
Chromosome:chromosome 8
Location:1998033
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGGAATTTAGTCGCCAAGGATGCCGACT
Internal bar code:GCCCTCCGGGATGTCACGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:99.5228
LEAP-Seq n confirming:14807
LEAP-Seq n nonconfirming:71
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACGATTGTTTGGTGCAA
Suggested primer 2:GGGAGGAGTGTGTGGAGTGT