| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139124 |
| Chromosome: | chromosome_8 |
| Location: | 1998033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367950 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCGGAATTTAGTCGCCAAGGATGCCGACT |
| Internal bar code: | GCCCTCCGGGATGTCACGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 99.5228 |
| LEAP-Seq n confirming: | 14807 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGATTGTTTGGTGCAA |
| Suggested primer 2: | GGGAGGAGTGTGTGGAGTGT |