Insertion junction: LMJ.RY0402.139124_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.139124
Chromosome:chromosome 8
Location:1998037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGTTGAGGAAAGCAGAATCGCCCTAG
Internal bar code:ACGGAATAGTCCTCTTACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:998
LEAP-Seq percent confirming:99.0071
LEAP-Seq n confirming:6980
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGAAGCAGTGGCAGTAG
Suggested primer 2:GGGAGGAGTGTGTGGAGTGT