| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139131 |
| Chromosome: | chromosome 7 |
| Location: | 1830344 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325730 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGAGCAGCTGCCAGACGGCGCCGTGGG |
| Internal bar code: | CCCCCGCACTCGTCAGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 90.7317 |
| LEAP-Seq n confirming: | 186 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAATGAGCTTGCGTCAAC |
| Suggested primer 2: | TGTCTGCGTATCTGCCTGTC |