| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139135 |
| Chromosome: | chromosome 1 |
| Location: | 4162947 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCTCCACGCCACACCGCTCCACTCCAA |
| Internal bar code: | TCAAGACCAGGGGGGACCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 95.9194 |
| LEAP-Seq n confirming: | 18523 |
| LEAP-Seq n nonconfirming: | 788 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGGTGGTAGCCGATAG |
| Suggested primer 2: | TAGACAGTGCATGAAAGGCG |