| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.139191 |
| Chromosome: | chromosome 10 |
| Location: | 1316864 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427550 | (1 of 3) IPR022751//IPR029044 - Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGCCCCCAGAGAGCTTCCGTAGCCAC |
| Internal bar code: | CAATCGACAGGGCGGTAAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.6862 |
| LEAP-Seq n confirming: | 18106 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACGGGATACCAGTCTAA |
| Suggested primer 2: | GAAGATGCAAGGCACTGACA |