| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139204 |
| Chromosome: | chromosome_4 |
| Location: | 1931500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g218250 | ARL3 | ARF-like GTPase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAAATGGAAACTTTGTTCGGACATTTAAC |
| Internal bar code: | CCTCACGGTTCACGACGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.1944 |
| LEAP-Seq n confirming: | 8988 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTAAACGTTGGTCCAG |
| Suggested primer 2: | GGCAGCTCCAAAGTGCTTAC |