Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.139213 |
Chromosome: | chromosome_9 |
Location: | 1264018 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g399400 | FAP199,TGL15 | Triacylglycerol lipase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTACTTCGTCCCCTCCATCAACTACTTTGG |
Internal bar code: | TTTCAGGTATCGAGGACAACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 694 |
LEAP-Seq percent confirming: | 99.2552 |
LEAP-Seq n confirming: | 6397 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTAGAAGGCGCAAGAGCTA |
Suggested primer 2: | CTTCACGTGAGTGTCTGCGT |