| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139216 |
| Chromosome: | chromosome 10 |
| Location: | 3697135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446050 | (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTACGTGCGGGAGTGGGTAGACCACCAC |
| Internal bar code: | AGCTAATGTTGGGCAGAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.598 |
| LEAP-Seq n confirming: | 28737 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTAGGACTACAACCCGC |
| Suggested primer 2: | CCCCGTGCATGAACTTACTT |