| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139228 |
| Chromosome: | chromosome 11 |
| Location: | 2572458 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476250 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGCACGTACTCGCTGTTGTACACGCCC |
| Internal bar code: | ACGTAATTCTTACTTATACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 99.7363 |
| LEAP-Seq n confirming: | 2269 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTTCGACTACCGCAACA |
| Suggested primer 2: | GAGGCGGTGTACTGGTAGGA |