| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139231 |
| Chromosome: | chromosome 5 |
| Location: | 3205041 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239800 | ARS14 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTGGTTGGGTTGGGCGGCGGGCCAGCG |
| Internal bar code: | GGGTAAGCAAAACTAGGCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 91.622 |
| LEAP-Seq n confirming: | 5643 |
| LEAP-Seq n nonconfirming: | 516 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAGGAGCAGGCAGTAAT |
| Suggested primer 2: | GACAACATACACCGCCCTCT |