| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139238 |
| Chromosome: | chromosome 9 |
| Location: | 2565085 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390700 | POB1 | Proteome of basal body 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGGCTGCAGCGGCAGGTCACGTTTGC |
| Internal bar code: | CACCGCACCCACTGAGCACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGTCCTCCTCTTCCTCT |
| Suggested primer 2: | TGCATAGCACAAGGGAAGTG |