| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139243 |
| Chromosome: | chromosome 3 |
| Location: | 160998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144164 | RSE2,RSEP2 | (1 of 1) PTHR22939:SF79 - PEPTIDASE M50 FAMILY PROTEIN; Intramembrane metalloprotease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGTGGGCGGCAAGTCACCGTGGATAG |
| Internal bar code: | ATCGGGCAGTCGCAGGTACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.3627 |
| LEAP-Seq n confirming: | 32741 |
| LEAP-Seq n nonconfirming: | 210 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCGGATCATGTGGTTCT |
| Suggested primer 2: | CCTTACGGGACAGGTTCAAA |