| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139265 |
| Chromosome: | chromosome 14 |
| Location: | 3234622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629283 | (1 of 1) 3.5.4.34 - tRNA(Ala)(adenine(37)) deaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCGGGACTCAGCCCCAGGGCCGCATCC |
| Internal bar code: | CCGTTGTCAGCCACTCGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 68.0476 |
| LEAP-Seq n confirming: | 4287 |
| LEAP-Seq n nonconfirming: | 2013 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTCAGTAAGCTGTGTT |
| Suggested primer 2: | AGTTGCGTGAGGTGTGTGAG |