| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139280 |
| Chromosome: | chromosome 3 |
| Location: | 5974723 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190500 | RSW3,GLH1,GLC2A | (1 of 1) 3.2.1.84 - Glucan 1,3-alpha-glucosidase / Exo-1,3-alpha-glucanase; Glucosidase IIa | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACTGTAGACCTTCATATCACGCGGCG |
| Internal bar code: | TCGCGCCCATGTGCAATTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.7543 |
| LEAP-Seq n confirming: | 406 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCTTGTGCATGGTGAT |
| Suggested primer 2: | GGTTTTAGCCCACATGCACT |