| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139307 |
| Chromosome: | chromosome 1 |
| Location: | 3104076 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019300 | Nucleotidyl cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGCAGCACTGTGGGCAGCGGTATGGTG |
| Internal bar code: | CCGATGTGTAGGTTGCGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 54.0033 |
| LEAP-Seq n confirming: | 661 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAAGCATAATGGTGGAG |
| Suggested primer 2: | GATGGTTGGTGTGCAAACAG |