| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139350 |
| Chromosome: | chromosome 6 |
| Location: | 8450066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307350 | (1 of 2) K12486 - stromal membrane-associated protein (SMAP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCGGCATGTGTGTGCGTGTGTGTGCG |
| Internal bar code: | TGGGTGGTCCACCCAGTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 80.8915 |
| LEAP-Seq n confirming: | 8856 |
| LEAP-Seq n nonconfirming: | 2092 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACTGGA |
| Suggested primer 2: | CAGAACAAGCATCACACCGT |