Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.139357 |
Chromosome: | chromosome_2 |
Location: | 5524777 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g108450 | FAP280 | Flagellar Associated Protein, transcriptional coactivator-like | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGAAGACACGCGCTCGTCTGCACGCAGGT |
Internal bar code: | CGACAGTGTAGGGACTTGGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1193 |
LEAP-Seq percent confirming: | 99.7512 |
LEAP-Seq n confirming: | 11226 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAAAGCATGGCTTACCCAC |
Suggested primer 2: | GGCTTCTCGTTGATAGCCTG |