| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.139374 |
| Chromosome: | chromosome 7 |
| Location: | 1683361 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325500 | MCH1,CHLH1 | Magnesium chelatase subunit H; (1 of 1) PTHR23304//PTHR23304:SF115 - SPOT2-RELATED // MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGCGGATGCAGAGAAGACCTTAGAGCA |
| Internal bar code: | GGCCGCGCACGGGAGTTCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1275 |
| LEAP-Seq percent confirming: | 99.3182 |
| LEAP-Seq n confirming: | 2622 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCTACTGGGCAGGCTAT |
| Suggested primer 2: | TACCAGCGTTTACTGACCCC |