| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139376 |
| Chromosome: | chromosome_3 |
| Location: | 7552156 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g205050 | DGTT4,CGLD24,DOT1 | Diacylglycerol O-acyltransferase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGAAAGGGGGAGGAGTAGTCTGGGGTCA |
| Internal bar code: | GGGTATCCGGGTTACCCGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 79.8743 |
| LEAP-Seq n confirming: | 10037 |
| LEAP-Seq n nonconfirming: | 2529 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGAATTCGCTTGCACA |
| Suggested primer 2: | TCTCCTTTGCGCTTTCCTAA |