Insertion junction: LMJ.RY0402.139389_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.139389
Chromosome:chromosome 5
Location:1378262
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g244400 (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAATACGCTGCACACTACGGTGAGCCAAC
Internal bar code:TCAGAGTCGGGCACCCCGCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:695
LEAP-Seq percent confirming:99.7261
LEAP-Seq n confirming:3641
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTACATGGATGTGTGCC
Suggested primer 2:ACGTAACTGATGGTTTCCGC