Insertion junction: LMJ.RY0402.139398_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139398
Chromosome:chromosome 2
Location:7040364
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g147450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACGAGCTTCGCACTTTCGTACTGCACA
Internal bar code:TGAGTCCGCGGAGTGTGAGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:514
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCAATTTGTTGAGCAGCG
Suggested primer 2:TTTACCGTTTCGCTGCTCTT